Essentiality is based on high through-put gene disruption studies. A gene is considered essential when no transposon insertion preventing the transcription could be found for its ORF, in the case of Mycoplasma mutants spp. In the case of Bacillus subtilis, a model organism for gram+ bacteria, selective knock-outs were performed by homologous recombination.
Genes found to be essential are indicated as 'yes', non-essential genes as 'no', and '-' is used if the respective gene has not been included in the analysis.
Mycoplasma pneumoniae: Hasselbring et al., 2006
Mycoplasma pulmonis: French et al., 2008
Mycoplasma genitalium: Glass et al., 2006
Bacillus subtilis: Kobayashi et al., 2003 |