MyMpn Project

Gene mpn133 (MPN133)

Name	mpn133
Stable ID	MPN133
Location	172277 - 173182 +
Sequence	1 ATGAAAGGGT TTTCTTGCTC CAGACCGGGC TATCTTACTG GTCTCTTGCT CCTCGCTGTA 61 GCCCCTATTC TCACTGCTTG TACCCGCGAC TATACTACCA AGAACGAATT TCAATTAACA 121 ACAGCTCAAC AGGCCAAATT AAAGCCAGCC ACCATCGAAT ATTGGCGTGA TGGTGATACG 181 CCTGAGATCA ATTACGCAAG CGAAGAGCGC CGAAAGGAAG CTGAACAAAA ATCTAAAGAA 241 AACGCTAAGA AAGAAGACAA AAAAGAGGAA AAGAAAACTG AAGATTCCCA AGATTCTTCT 301 TCTGCATCCA CGCAAGTTCG TAGTAGTAAA CACGGTCTCC GCATCTATGG AATAGATACA 361 CCCGAAAAAC ATGTAAGTAG CAAAGGCGAT AGCACCGGTG ATGAAAAAAT CGAAGCCGAA 421 AAGGCCTCTA ACTATGCTGA AAAGCTAATC CCTAAAGGCA GTACTGTTTG GGTTTGATCA 481 CTCAACACTT ACAGCTATGA TCGTGAAGTG GGCGCTTTAT TCTTTAAGTC TAACCCTAAG 541 CAAACCTTCT TCCAATCCTT TGAAGTAGCT ATGGTTGAGG CTGGTCATGC TATTCCAATT 601 GCTGGGACTG GTTTAAATTT AATCGCTGAT CCGGAATTAA GTGCTGATGA TCCTTTAAGT 661 GTCATTGGTC TACAGTTAGC CAACGCTGCC AATAAGGCTT ACAACGCTAA AATTAACATT 721 TGGAGCCATG ACACTGATGG TTATCGTTCC TTGACTGCTG TTTACAAGTT AAGGGGCGCT 781 GATATTAGTT GAACCCGTTT CCTAGATGAA GCCAATGGAT ATTCCTCTGC TAGTGCTGGA 841 ACCGGTGCTA GTTTGTACCA ACTATGAGAT CAACGTCAAG CCAAATTAGC CCAAAAGGGT 901 AGTTAA
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Operon	OP64
Operon location	172232 - 177000

Protein (mpn133)

Name	Ca2+-dependent cytotoxic nuclease of Mycoplasma
Stable ID	Mpn133
Molecular Weight	33110
Isoelectric Point	7
Localization	LIPOP
Comment	We identified Mpn133 as a Ca2+-dependent cytotoxic nuclease of Mycoplasma injected into the host cell. important contributor to M. pneumoniae-associated life cycle events and as a virulence factor in host-associated cytopathologies. In addition, the distinct property of the EKS peptide in delivery of proteins, like mCherry, into target cells opens new avenues to the establishment of novel concepts of drug delivery and therapy.
Sequence	MKGFSCSRPGYLTGLLLLAVAPILTACTRDYTTKNEFQLTTAQQAKLKPATIEYWRDGDTPEINYASEERRKEAEQKSKE NAKKEDKKEEKKTEDSQDSSSASTQVRSSKHGLRIYGIDTPEKHVSSKGDSTGDEKIEAEKASNYAEKLIPKGSTVWVWS LNTYSYDREVGALFFKSNPKQTFFQSFEVAMVEAGHAIPIAGTGLNLIADPELSADDPLSVIGLQLANAANKAYNAKINI WSHDTDGYRSLTAVYKLRGADISWTRFLDEANGYSSASAGTGASLYQLWDQRQAKLAQKGS

Post translational modifications

No post translational modifications were found

GENE/PROTEIN mpn133 (Domains Overview)

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Domains (InterProScan)

Location (aa)	Name (InterPro ID)	Description	GO terms	Sequence	Evalue
1-27	PS51257 (n.a.)	NULL			5
39-244	SSF50199 (IPR016071)	Staphylococcal nuclease (SNase-like), OB-fold	Molecular Function: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003676'>'nucleic' == '' ? '': 'nucleic'; 'acid' == '' ? '': 'acid'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003676) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:'hydrolase' == '' ? '': 'hydrolase';'>'hydrolase' == '' ? '': 'hydrolase'; 'activity' == '' ? '': 'activity';		0.00000002
46-243	SM00318 (IPR006021)	Staphylococcal nuclease (SNase-like)	Molecular Function: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003676'>'nucleic' == '' ? '': 'nucleic'; 'acid' == '' ? '': 'acid'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003676) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:'hydrolase' == '' ? '': 'hydrolase';'>'hydrolase' == '' ? '': 'hydrolase'; 'activity' == '' ? '': 'activity';		0.00000000047
46-243	PS50830 (IPR006021)	Staphylococcal nuclease (SNase-like)	Molecular Function: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003676'>'nucleic' == '' ? '': 'nucleic'; 'acid' == '' ? '': 'acid'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003676) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:'hydrolase' == '' ? '': 'hydrolase';'>'hydrolase' == '' ? '': 'hydrolase'; 'activity' == '' ? '': 'activity';		19.159
65-95	coil (n.a.)	NULL	n.a.		0
77-101	seg (n.a.)	NULL	n.a.		0
108-243	G3DSA:2.40.50.90 (IPR006021)	Staphylococcal nuclease (SNase-like)	Molecular Function: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003676'>'nucleic' == '' ? '': 'nucleic'; 'acid' == '' ? '': 'acid'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003676) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:'hydrolase' == '' ? '': 'hydrolase';'>'hydrolase' == '' ? '': 'hydrolase'; 'activity' == '' ? '': 'activity';		0.000017
227-240	seg (n.a.)	NULL	n.a.		0
271-284	seg (n.a.)	NULL	n.a.		0

Protein Homology (BLASTP results)

Protein Start	Protein End	Hit Size	Orthologous	Organism	Orthologous Start	Orthologous End	Evalue	Identity	Score	Alignment
113	199	86	- MARTH_orf834	Mycoplasma arthritidis	127	211	0.0000007	37.0787	107
35	211	176	- MAG5040	Mycoplasma agalactiae	167	315	0.00000003	27.957	118
113	211	98	- MCJ_000150	Mycoplasma conjunctivae	124	224	0.000002	30.4762	104
12	268	256	- MYCGA0330	Mycoplasma gallisepticum	9	247	0.000000000000003	23.8267	180
1	268	267	- MG186	Mycoplasma genitalium	1	225	<1e-50	36.9403	473
113	211	98	- MHP7448_0368	Mycoplasma hyopneumoniae 7448	126	228	0.000000001	33.0189	132
113	198	85	- MHO_0730	Mycoplasma hominis	132	212	0.00000002	42.0455	120
113	199	86	-	Mycoplasma pulmonis	89	172	0.0000000008	40.9091	133

External IDs

COG	COG1525L
Gene ID	877227
GI	13507872
GO	DNA replication, recombination and repair
Home COG	L
InterPro	IPR002071\|Thermonuclease active site
InterPro	IPR006021\|Staphylococcus nuclease (SNase-like)
Old MP number	MP021
Pathway	Membrane associated
PDB homologs	1ey7_A
PDB homologs	1sty_
PDB homologs	1f2m_A
Pfam	PF00565
PID	g1673668
RefSeq	NP_109821.1
Swiss-Prot protein ID	Y133_MYCPN
phylomeDB tree	Y133_MYCPN
UniProt	P75265

Transcription

IMAGE BROWSERS

OPERON OP64 (Genomic Overview)

Region:172232-177000

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STRING image

STRING legend

PDB image(s)

PDB 1ey7

PDB 1sty

PDB 1f2m