MyMpn
The Mycoplasma pneumoniae database
 
e.g Mpn001 or dnaN or polymerase
Gene yacA (MPN222)
Name 
yacA
Stable ID 
MPN222
Location 
273701 - 274570 +
Sequence 
    1  ATGCCTAAAA CACAATACAT AGCTGGAGTA TCAGGTGGTC CTGATTCAAT GTTGTTGTTA
   61  AAACTGTACC ACAAGAAGAT TGCCTGTGTT GTACACGTTA ACTACAATAA GCGGACTACT
  121  GCACTCCGCG ACCAAAAAAT GGTCGAAGAA TACTGTAAAC AGCTCAAAGT GCCCCTCATT
  181  GTGCACACGG TACCTGAAGA TACCGTTTGA AAGAAAAACT TTCAAGCACA AGCTAGAAAA
  241  ATTCGCTTTG AACAGTTTCA AAAAGCAGCT AGCCTTTATA AAGTAGATAA GCTGCTGTTA
  301  GCCCACCACC GCGATGATTT TATCGAACAG GCCAAAATGC AGTTGGACGC ACGCAAGCGT
  361  GCCATGTATT ATGGCATTAA AACACGTGGC GAATTGTATG GGATGAAGGT CTATCGTCCC
  421  TTTATTAAGT ACTGAAAAAA CGAGATCTTA GCCTTGTGTG AAGAACACAA AGTGCCTTAT
  481  GGTATTGATG AAACCAACGC GCAGCCAATT TACAAACGTA ATCAGGTACG CCAGGAGATC
  541  GCTAACTGAT CGAAGGAAGA GAAGGAGGAG TTTTACATTG GTGTGTGCGC CATGAACCGC
  601  GTCATTGGTC AAAAGCTCTT TTCCTTAATG AAACGTGCAA AACAGTGGTT AGCTCATCCT
  661  GATGTACGTG AATTAAAGCG TTACCCACTT CCCGATCAAC GTCAGTTGGT TTACAGCTTT
  721  TTAATTACCC ACCACATTGA TGTAACGGGG GACAAGATTG ATGCCATTTT AGACTTTATC
  781  CAACCGTTTC AACAAAAGCA TTACCGTCTC AAGGATGAGA TCTTCTTATC CATTAAAGAC
  841  GAACGGCTAA CTTTACTGTA CAAGAGTTAG
Download Sequence
Operon 
OP93
Operon location 
265662 - 273150
Protein (mpn222)
Name 
tRNA(Ile)-lysidine synthase (EC 6.3.4.-) (tRNA(Ile)-lysidine synthetase) (tRNA(Ile)-2-lysyl-cytidine synthase)
Stable ID 
Mpn222
Molecular Weight 
31790
Isoelectric Point 
10
Localization 
Cytoplasm
Comment Also tilS. Denote as cell cycle protein MesJ, but this is a misnomer. Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (Molecular cell, 2003, 12:689)
Sequence 
MPKTQYIAGVSGGPDSMLLLKLYHKKIACVVHVNYNKRTTALRDQKMVEEYCKQLKVPLIVHTVPEDTVWKKNFQAQARK
IRFEQFQKAASLYKVDKLLLAHHRDDFIEQAKMQLDARKRAMYYGIKTRGELYGMKVYRPFIKYWKNEILALCEEHKVPY
GIDETNAQPIYKRNQVRQEIANWSKEEKEEFYIGVCAMNRVIGQKLFSLMKRAKQWLAHPDVRELKRYPLPDQRQLVYSF
LITHHIDVTGDKIDAILDFIQPFQQKHYRLKDEIFLSIKDERLTLLYKS
Post translational modifications
Modification Modified sequence Relative start Relative end Amino acid
Oxidation PKTQYIAGVSGGPDSmLLLK 2 22 M
GENE/PROTEIN yacA (Domains Overview)
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Domains (InterProScan)
Location (aa) Name (InterPro ID) Description GO terms Sequence Evalue
2-209 SSF52402 (n.a.) NULL 2.3e-38
5-163 G3DSA:3.40.50.620 (IPR014729) Rossmann-like alpha/beta/alpha sandwich fold 3.8e-16
5-182 TIGR02432 (IPR012795) Lysidine-tRNA(Ile) synthetase, N-terminal Biological Process:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006412'>'translation' == '' ? '': 'translation'; ('GO' == '' ? '': 'GO';:0006412) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008033'>'tRNA' == '' ? '': 'tRNA'; 'processing' == '' ? '': 'processing'; ('GO' == '' ? '': 'GO';:0008033)

Cellular Component:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0005737'>'cytoplasm' == '' ? '': 'cytoplasm'; ('GO' == '' ? '': 'GO';:0005737)

Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0000166'>'nucleotide' == '' ? '': 'nucleotide'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0000166) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0005524'>'ATP' == '' ? '': 'ATP'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0005524) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:'ligase' == '' ? '': 'ligase';'>'ligase' == '' ? '': 'ligase'; 'activity' == '' ? '': 'activity';

0
6-179 PF01171 (IPR011063) tRNA(Ile)-lysidine/2-thiocytidine synthase 0
13-180 PTHR11807:SF2 (IPR012094) Lysidine-tRNA(Ile) synthetase Biological Process:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006412'>'translation' == '' ? '': 'translation'; ('GO' == '' ? '': 'GO';:0006412) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008033'>'tRNA' == '' ? '': 'tRNA'; 'processing' == '' ? '': 'processing'; ('GO' == '' ? '': 'GO';:0008033)

Cellular Component:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0005737'>'cytoplasm' == '' ? '': 'cytoplasm'; ('GO' == '' ? '': 'GO';:0005737)

Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0000166'>'nucleotide' == '' ? '': 'nucleotide'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0000166) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0005524'>'ATP' == '' ? '': 'ATP'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0005524) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:'ligase' == '' ? '': 'ligase';'>'ligase' == '' ? '': 'ligase'; 'activity' == '' ? '': 'activity';

2.2e-31
13-180 PTHR11807 (n.a.) NULL 2.2e-31
Protein Homology (BLASTP results)
Protein Start Protein End Hit Size Orthologous Organism Orthologous Start Orthologous End Evalue Identity Score Alignment
5 177 172 - b0188 Escherichia coli 14 191 0.000000002 26.2295 134
3 286 283 - MARTH_orf854 Mycoplasma arthritidis 16 305 2.99878e-43 33.4471 420
7 285 278 - MAG7460 Mycoplasma agalactiae 3 286 2e-36 31.5789 362
2 275 273 - MCJ_003070 Mycoplasma conjunctivae 5 280 2e-35 33.5714 354
5 219 214 - MCAP_0014 Mycoplasma capricolum subsp. capricolum 11 223 4e-32 34.0909 325
3 276 273 - MYCGA6670 Mycoplasma gallisepticum 6 275 <1e-50 37.5451 508
5 289 284 - MG084 Mycoplasma genitalium 6 290 <1e-50 71.9298 1144
2 177 175 - MHP7448_0205 Mycoplasma hyopneumoniae 7448 7 182 5e-32 35.9551 324
5 276 271 - MHO_4220 Mycoplasma hominis 27 301 1e-35 30.8244 354
5 180 175 - MSC_0042 Mycoplasma mycoides subsp. mycoides SC 11 191 2e-30 37.0166 310
3 283 280 - MYPE1460 Mycoplasma penetrans 2 290 2e-36 31.6151 364
4 206 202 - Lactococcus lactis subsp. lactis KF147 22 220 0.0000000000007 27.1429 162
4 288 284 - MS53_0615 Mycoplasma synoviae 3 289 5e-37 33.3333 366
7 192 185 - Bacillus subtilis subsp. subtilis 35 230 9e-20 33.3333 226
7 192 185 - Bacillus subtilis subsp. subtilis 21 216 2e-19 33.3333 224
7 276 269 - Mycoplasma pulmonis 3 274 3.00004e-41 35.1449 404
External IDs
Alias 
tilS
COG 
COG0037D
EC number 
6.3.4.-
Gene ID 
877151
GI 
13507961
GO 
Translation, ribosomal structure and biogenesis
Home COG 
J
InterPro 
IPR012094|Cell cycle control PP-loop ATPase MesJ/YaeO
InterPro 
IPR011063|PP-loop
InterPro 
IPR012795|tRNA(Ile)-lysidine synthetase, N-terminal
Old MP number 
MP609
Pathway 
tRNA metabolism
PDB homologs 
1GPM-A
Pfam 
PF01171
PID 
g1674311
RefSeq 
NP_109910.1
Swiss-Prot protein ID 
TILS_MYCPN
phylomeDB tree 
TILS_MYCPN
UniProt 
P75549
Transcription
IMAGE BROWSERS

OPERON OP93 (Genomic Overview)
Region:265662-273150

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STRING image

STRING of Mpn222STRING legend

PDB image(s)

1GPM

PDB 1GPM
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