Gene trmB (MPN522)
Name
trmB
Stable ID
MPN522
Location
643251 - 643883 +
Sequence
    1  GTGCGCTTAA GAAAAGTTAA AGATGCTCTT GTAAGGGTAC AAGACAGCCC TTACTTTAGT
   61  AACCCAGAAC AACTGTCAAC TATTGAAAGT GAATCGCTCT TCGTGGAAAT AGGTTGTGGT
  121  AAAAGTTGGT TTTTAAGCCA GCAAGCACAG CAAAACCCAA AGTGTTTTTT TGTGGGAATC
  181  GAGCGTGAAC CCACGATTGT TTTAAAGGCA ATTAATAAGG TAAACCGCTT AGAGGTTAAA
  241  CCACGTAATT TATTAATAAC GTGTTTGGAT GCCAACCAGC TAACAGAGTA CTTAAAACCA
  301  CAATCGATTA GCAAAATCTT CATTAACTTC CCCGATCCTT GACCAAAAAA GCGTCACACG
  361  TTGAGGCGAC TAACTGCTCC ACATTTCCTC AAGCAGTTTC ACCAACTTTT AAAAAAGCAA
  421  GGTTTAATAG AATTCAAAAC GGACAACGAT CAGTTGTTTG AATTTACCTT AGAAGTCTTG
  481  CAGAAAACAG TAGCTCACTT TAAGATAATT GAGCAAACGA CAGATCTGCA CAATTCACCT
  541  TTGACTAGTA CCAATATTAT GACCGAATAC GAACAGCGCT TTGTTAGTTT AGGGGTTAAG
  601  ATTAAAAAGC TAACGCTTGA AAAACTAAAT TAG
Download Sequence
Operon
OP220
Operon location
642770 - 643900
Protein (mpn522)
Name
tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33) (tRNA(m7G46)-methyltransferase)
Stable ID
Mpn522
Molecular Weight
23100
Isoelectric Point
10
Localization
Cytoplasm
Comment tRNA modification position 46. G->m7G (7-methylguanosine); cofactor: SAM
Sequence
MRLRKVKDALVRVQDSPYFSNPEQLSTIESESLFVEIGCGKSWFLSQQAQQNPKCFFVGIEREPTIVLKAINKVNRLEVK
PRNLLITCLDANQLTEYLKPQSISKIFINFPDPWPKKRHTLRRLTAPHFLKQFHQLLKKQGLIEFKTDNDQLFEFTLEVL
QKTVAHFKIIEQTTDLHNSPLTSTNIMTEYEQRFVSLGVKIKKLTLEKLN
Post translational modifications
No post translational modifications were found
GENE/PROTEIN trmB (Domains Overview)
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Domains (InterProScan)
Location (aa) Name (InterPro ID) Description GO terms Sequence Evalue
1-203 PTHR23417 (n.a.) NULL 3.2e-30
1-203 PTHR23417:SF1 (IPR003358) tRNA (guanine-N-7) methyltransferase Biological Process:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006400'>'tRNA' == '' ? '': 'tRNA'; 'modification' == '' ? '': 'modification'; ('GO' == '' ? '': 'GO';:0006400)

Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008176'>'tRNA' == '' ? '': 'tRNA'; ('guanine' == '' ? '': 'guanine';-'N' == '' ? '': 'N';7-)-'methyltransferase' == '' ? '': 'methyltransferase'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0008176)

3.2e-30
4-207 SSF53335 (n.a.) NULL 2.2e-24
17-210 TIGR00091 (IPR003358) tRNA (guanine-N-7) methyltransferase Biological Process:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006400'>'tRNA' == '' ? '': 'tRNA'; 'modification' == '' ? '': 'modification'; ('GO' == '' ? '': 'GO';:0006400)

Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008176'>'tRNA' == '' ? '': 'tRNA'; ('guanine' == '' ? '': 'guanine';-'N' == '' ? '': 'N';7-)-'methyltransferase' == '' ? '': 'methyltransferase'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0008176)

0
21-208 PF02390 (IPR003358) tRNA (guanine-N-7) methyltransferase Biological Process:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006400'>'tRNA' == '' ? '': 'tRNA'; 'modification' == '' ? '': 'modification'; ('GO' == '' ? '': 'GO';:0006400)

Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008176'>'tRNA' == '' ? '': 'tRNA'; ('guanine' == '' ? '': 'guanine';-'N' == '' ? '': 'N';7-)-'methyltransferase' == '' ? '': 'methyltransferase'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0008176)

0
31-182 G3DSA:3.40.50.150 (n.a.) NULL 0.000000098
Protein Homology (BLASTP results)
Protein Start Protein End Hit Size Orthologous Organism Orthologous Start Orthologous End Evalue Identity Score Alignment
1 208 207 - MAG6780 Mycoplasma agalactiae 1 203 7e-34 36.0577 338
1 201 200 - MARTH_orf090 Mycoplasma arthritidis 1 194 8e-34 39.801 338
29 193 164 - Caulobacter crescentus NA1000 52 211 5e-19 29.0909 216
1 194 193 - MCJ_000410 Mycoplasma conjunctivae 1 186 3e-31 37.4359 316
1 204 203 - Lactococcus lactis subsp. lactis KF147 1 209 2e-29 32.8638 304
33 207 174 - Bacillus subtilis subsp. subtilis 39 208 3e-36 40.678 366
33 207 174 - Bacillus subtilis subsp. subtilis 41 210 4e-36 40.678 366
35 209 174 - b2960 Escherichia coli 68 238 0.0000000000003 28.4091 166
1 197 196 - MG347 Mycoplasma genitalium 1 197 <1e-50 57.3604 606
33 208 175 - Rv0208c Mycobacterium tuberculosis 79 252 0.00000000008 25.1397 145
35 209 174 - MCAP_0761 Mycoplasma capricolum subsp. capricolum 41 219 2e-29 39.4444 300
1 196 195 - MS53_0113 Mycoplasma synoviae 1 190 5e-32 40.6091 322
1 204 203 - MHP7448_0465 Mycoplasma hyopneumoniae 7448 1 196 2e-37 40.9756 369
35 204 169 - MMOB4960 Mycoplasma mobile 33 197 5e-32 41.7647 322
35 209 174 - MSC_0166 Mycoplasma mycoides subsp. mycoides SC 42 220 8e-26 37.5691 270
31 148 117 - LIC_10126 Leptospira interrogans serovar Copenhage 43 159 0.000000000002 32.7731 158
1 207 206 - MYCGA3170 Mycoplasma gallisepticum 4 214 3e-38 38.4977 377
2 207 205 - MYPE2880 Mycoplasma penetrans 3 215 5e-32 35.814 324
1 201 200 - Mycoplasma pulmonis 1 194 1e-31 40.3846 321
1 201 200 - MHO_4250 Mycoplasma hominis 1 194 1e-29 35.8209 302
External IDs
COG
COG0220R
EC number
2.1.1.33
Gene ID
876965
GI
13508261
GO
Translation, ribosomal structure and biogenesis
Home COG
J
InterPro
IPR004395|Conserved hypothetical protein 91
InterPro
IPR003358|Putative methyltransferase
Old MP number
MP320
Pathway
tRNA metabolism
PDB homologs
1XVA-A
PDB homologs
1ve3
PDB homologs
1p91_A
PDB homologs
1f38_A
Pfam
PF02390
PID
g1673993
RefSeq
NP_110210.1
Swiss-Prot protein ID
TRMB_MYCPN
phylomeDB tree
TRMB_MYCPN
UniProt
P75256
Transcription
IMAGE BROWSERS

OPERON OP220 (Genomic Overview)
Region:642770-643900

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STRING image

STRING of Mpn522STRING legend

PDB image(s)

1XVA

PDB 1XVA

1ve3

PDB 1ve3

1p91

PDB 1p91

1f38

PDB 1f38