MyMpn Project

Comparison with some other bacteria

Metabolites	M. pneumoniae	E. coli	B.subtilis	L.lactis
average Fructose-1,6-bisphosphate in mM	0.34 (SD=7.2%)	2.95 (Lowry et al 1971)		50 (Papagianni 2007)
average Glycerol-3-phosphate in mM	0.19 (SD=16.2%)	0.195 (bionumbers)		36.6 (starvation; Neves 2002)
average Phosphoenolpyruvate in mM	0.11 (SD=0.9%)	2.73 (Lowry et al 1971) 0.091-0.3 (Bionumbers)	<0.02 (Fry 2000)	11 (Papagianni 2007)
average Glucose-6-phosphate in mM	0.09 (SD=7.1%)	0.04956 (Lowry et al 1971) 0.801 (Bionumbers)	<0.1 (Fry 2000)	20 (Papagianni 2007)
average Fructose-6-phosphate in mM	0.08 (SD=1.0%)			23 (Papagianni 2007)
average Ribose-5-phosphate in mM	0.03 (SD=8.2%)
average Dihydroxy-acetone-phosphate in mM	0.10 (SD=50%)	0.203 (bionumbers)
average ATP concentration in mM	4.1[Krebes 1995]	2.6 (bionumbers), 1.3-7.0 (CCBD)		6 (Papagianni 2007)

Comparison between Mycoplasma pneumoniae and some other bacteria

Species	Taxonomy	Genome size (Mb)	ORFs	%G+C	rRNA operons	>ribosomes per cell	Doubling time (hours)	Dimensions (microns)	Cell volume (microns³)	Life style	Culture density (cells/ml)
Escherichia coli	Proteobacteria / Gamma proteobacteria	4.6	4489	50.8	7	7000-50000	0.33	0.8 * 1-3	1.0048	Mutualist / pathogen	10^8
Salmonella typhimurium	Proteobacteria / Gamma proteobacteria	4.85	4423	52	7	?	1.3	1 * 2-3	1.9625	Free living/ pathogen	10^9
Haemophilus influenzae	Proteobacteria / Gamma proteobacteria	1.91	1792	38	6	50000	0.5	0.43	0.04160866	Pathogen	10^10
Pseudomonas aeruginosa	Proteobacteria / Gamma proteobacteria	6.6	5925	66	4	?	1.5	0.5-1.0 * 1-5	1.10390625	Pathogen	10^8
Yersinia pestis	Proteobacteria / Gamma proteobacteria	4.65	4198	47.64	6	?	2	0.7 * 2	0.7693	Pathogen	10^8
Burkholderia pseudomallei	Proteobacteria / Beta Proteobacteria	7.24	6347	68	4	?	1.5-2.3	0.4-0.8 * 2-5	0.9891	Free living/ pathogen	10^8
Caulobacter crescentus	Proteobacteria / Alphaproteobacteria	4.01	3737	67	2	?	1.15	0.7 * 2-3	0.576975	Free living	10^8
Pelagibacter ubique	Proteobacteria / Alphaproteobacteria	1.3	1354	29	1	?	1.6	0.2 * 0.4	0.01256	Free living	10^6
Rickettsia prowazekii	Proteobacteria / Alphaproteobacteria	1.11	834	29	1	1500	10	0.3-0.5 * 0.8-2	0.17584	Pathogen	10^7
Mycobacterium tuberculosis	Actinobacteria / Actinobacteria	4.4	3924	65	1	4000	24	0.4 * 1-2	0.1884	Pathogen	10^8?
Mycobacterium leprae	Actinobacteria / Actinobacteria	3.26	1605 (2720)	57.8	1	5000	336	0.4 * 1-2	0.1884	Pathogen	14*10^7?
Borrelia recurrentis	Spirochaetes / Spriochaetes	1.24	990	27.5	3	20000?	12	0.8 * 1.8	0.90432	Pathogen	?
Staphylococcus aureus	Firmicutes / Cocci	2.8	2900	32.8	5	83000	0.5	1	0.52333333	Pathogen	10^9
Bacillus subtilis	Firmicutes / Bacilli	4.2	4100	43	10	6000	0.4	0.7-0.8 * 2-3	1.10390625	Free living	10^8
Lactococcus lactis	Firmicutes / Bacilli	2.36	2300	35	6	?	0.4-0.8	0.5-1	0.22078125	Free living	10^8
Clostridium botulinum	Firmicutes / Clostridia	3.86	3548	28	8	?	5	0.5-0.8 * 3-8	0.6908	Pathogen	10^8
Mycoplasma penetrans	Firmicutes / Mollicutes	1.36	1038	25	1	?	?	0.3 * 1.4	0.09891	Pathogen	10^8?
Mycoplasma gallisepticum	Firmicutes / Mollicutes	0.99	726	31.45	1	?	2-3	0.5 * 0.7	0.137375	Pathogen	3 * 10^8
Mycoplasma pneumoniae	Firmicutes / Mollicutes	0.81	686	40	1	300	8	0.4 * 0.6	0.07536	Pathogen	10^9
Mycoplasma genitalium	Firmicutes / Mollicutes	0.58	479	31.7	1	?	12	0.3 * 0.5	0.035325	Pathogen	>10^7
Thermus aquaticus	Euryarchaeota / Thermococci	2.08	2306	51	1	?	1	0.5-0.8 * 5-10	2.393622	Free living	10^9
Thermococcus kodakaraensis	Euryarchaeota / Thermococci	2.08	2358	52	1	?	0.9	1-2	1.76625	Free living	10^8

References:

Bionumbers
CCBD
Edwards JS and Palsson BO (2000). The Escherichia coli MG1655 in silico metabolic genotype: its definition, characteristics, and capabilities. Proc Natl Acad Sci USA 97:5528-5533.
Krebes, K. A., Dirksen, L. B. and Krause, D. C. (1995). Phosphorylation of Mycoplasma pneumoniae cytadherence-accessory proteins in cell extracts. J Bacteriol 177, 4571-4.
Lowry OH, Carter J, Ward JB, Glaser L (1971). The effect of carbon and nitrogen sources on the level of metabolic intermediates in Escherichia coli. J Biol Chem 246:6511-6521.
Natarajan A and Srienc F (2000). Glucose uptake rates of single E. coli cells grown in glucose-limited chemostat cultures. J Microbiol Methods 42:87-96.
Selinger DW, Saxena RM, Cheung KJ, Church GM, Rosenow C (2003). Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation. Genome Res 13:216-223.