To isolate intracellular metabolites, cells were washed thoroughly with PBS containing 0.05% glucose. Cells were lysed and Metabolism was quenched by adding 5-10 ml of -80ÂșC methanol directly to the culture dishes with attached cells. Lysates were collected, cell debris removed by centrifugation and supernatants were frozen in liquid nitrogen and lyophilyzed. Freeze-dried samples were processed according to analytic requirements (see Maier et al., 2013) and analysed by GC-MS, LC-MS or NMR.

Metabolite name KEGG ID Chemical sum formula Molecular weight Identified by
(S)-lactate C00186 C3H6O3 90.08 NMR
3-phospho-D-glycerate C00197 C3H7O7P 186.06 LC-MS
acetate C00033 C2H4O2 60.05 NMR
adenine C00147 C5H5N5 135.14 GC-MS
adenosine C00212 C10H13N5O4 267.25 GC-MS
ADP C00008 C10H15N5O10P2 427.21 LC-MS, NMR
ADP-glucose C00498 C16H25N5O15P2 589.35 LC-MS
alanine C00041 C3H7NO2 89.1 GC-MS
AMP C00020 C10H14N5O7P 347.23 LC-MS
arginine C00062 C6H14N4O2 174.21 GC-MS, NMR
asparagine C00152 C4H8N2O3 132.12 GC-MS
aspartate C00049 C4H7NO4 133.11 GC-MS, NMR
ATP C00002 C10H16N5O13P3 507.19 LC-MS, NMR
CDP C00112 C9H15N3O11P2 404.19 LC-MS
cholesterol C00187 C27H46O 386.64 GC-MS
choline C00114 C5H14NO 104.17 NMR
CMP C00055 C9H14N3O8P 323.2 LC-MS
CTP C00063 C9H16N3O14P3 483.16 LC-MS
cysteine C00097 C3H7NO2S 121.17 GC-MS
cytosine C00380 C4H5N3O 111.11 GC-MS
D-fructose 1,6-bisphosphate C00354 C6H14O12P2 340.11 LC-MS, GC-MS
D-glucose 1-phosphate C00103 C6H13O9P 260.13 LC-MS, GC-MS
D-glucose 6-phosphate C00092 C6H13O9P 260.13 LC-MS, GC-MS
D-mannitol 1-phosphate C00644 C6H15O9P 262.15 LC-MS
dADP C00206 C10H15N5O9P2 411.21 LC-MS
dAMP C00360 C10H14N5O6P 331.23 LC-MS
dATP C00131 C10H16N5O12P3 491.19 LC-MS
dGDP C00361 C10H15N5O10P2 427.21 LC-MS
dGMP C00362 C10H14N5O7P 347.23 LC-MS
dGTP C00286 C10H16N5O13P3 507.19 LC-MS
dihydroxyacetone phosphate C00111 C3H7O6P 170.06 GC-MS
ethanol C00469 C2H6O 46.07 NMR
FAD C00016 C27H33N9O15P2 785.56 LC-MS
fatty acids (various) - - 0 GC-MS
formate C00058 CH2O2 46.03 NMR
fructose C00095 C6H12O6 180.16 GC-MS
fructose 6-phosphate C00085 C6H13O9P 260.13 GC-MS
fumarate C00122 C4H4O4 116.07 NMR
glyceraldehyde 3-phosphate C00118 C3H7O6P 170.06 GC-MS
GDP C00035 C10H15N5O11P2 443.21 LC-MS
glucose C00031 C6H12O6 180.16 GC-MS
glutamate C00025 C5H9NO4 147.13 GC-MS, NMR
glutamine C00064 C5H10N2O3 146.15 GC-MS
glycerol C00116 C3H8O3 92.09 GC-MS
glycerol 3-phosphate (sn-Glycerol 3-phosphate) C00093 C3H9O6P 172.07 LC-MS, GC-MS
glycine C00037 C2H5NO2 74.06 GC-MS, NMR
GMP C00144 C10H14N5O8P 363.23 LC-MS
GTP C00044 C10H16N5O14P3 523.19 LC-MS
guanine C00242 C5H5N5O 151.14 GC-MS
guanosine C00387 C10H13N5O5 283.25 GC-MS
histidine C00135 C6H9N3O2 155.16 GC-MS, NMR
isoleucine C00407 C6H13NO2 131.17 GC-MS, NMR
leucine C00123 C6H13NO2 131.17 GC-MS, NMR
lysine C00047 C6H14N2O2 146.19 GC-MS, NMR
methionine C00073 C5H11NO2S 149.22 GC-MS, NMR
NAD+ C00003 C21H28N7O14P2 664.44 LC-MS, NMR
NADH C00004 C21H29N7O14P2 665.45 LC-MS
NADP+ C00006 C21H29N7O17P3 744.42 LC-MS
NADPH C00005 C21H30N7O17P3 745.43 LC-MS
ornithine C00077 C5H12N2O2 132.17 NMR
phenylalanine C00079 C9H11NO2 165.19 GC-MS, NMR
phosphoenolpyruvate C00074 C3H5O6P 168.04 GC-MS
proline C00148 C5H9NO2 115.13 GC-MS, NMR
pyruvate C00022 C3H4O3 88.06 NMR
riboflavin C00255 C17H20N4O6 376.37 LC-MS
ribose C00121 C5H10O5 138.12 GC-MS
ribose-5-phosphate C00199 C5H11O8P 230.11 GC-MS
serine C00065 C3H7NO3 105.1 GC-MS, NMR
succinate C00042 C4H6O4 118.09 NMR
thiamine diphosphate C00068 C12H19N4O7P2S 425.32 LC-MS
threonine C00188 C4H9NO3 119.12 GC-MS, NMR
thymidine-5'-diphosphate C00363 C10H16N2O11P2 402.19 LC-MS
thymidine-5'-phosphate C00364 C10H15N2O8P 322.21 LC-MS
thymidine-5'-triphosphate C00459 C10H17N2O14P3 482.17 LC-MS
thymine C00178 C5H6N2O2 126.12 GC-MS
trans-4-hydroxy-L-proline C03651 C5H9NO3 131.13 NMR
tryptophane C00078 C11H12N2O2 204.23 GC-MS, NMR
tyrosine C00082 C9H11NO3 181.19 GC-MS, NMR
UDP C00015 C9H14N2O12P2 404.16 LC-MS
UDP-D-galactose C00052 C15H24N2O17P2 566.3 LC-MS, NMR
UDP-D-glucose C00029 C15H24N2O17P2 566.3 LC-MS, NMR
UMP C00105 C9H13N2O9P 324.18 LC-MS
uracil C00106 C4H4N2O2 112.09 GC-MS
uridine C00299 C9H12N2O6 244.21 GC-MS
UTP C00075 C9H15N2O15P3 484.14 LC-MS
valine C00183 C5H11NO2 117.15 GC-MS, NMR