MyMpn Project

1 ATGGAACAAC TTTTAGACCA GTTTTTAAGT GGTGTTTTAA GCGAATGGAA GGCTTTTATT 61 TTGCAAGAAG CCAAACAGAC CTATTTTCAA GAATTGTTAG CTAAACTAAA AAATGTCGAG 121 CAAGAGCTAA CTCCCAAAGC CTCTCAAGTC TTTCGTCCCT TTAGCTTTTT TGCACCAAAT 181 AACACTAAAC TAATTATTTA CGGTCAGGAT CCATATCCTA ATCCACAACA CGCTTGTGGT 241 CTTTCGTTTG CCTCTAACGC CCCTAAACTA CCACAAAGCT TAAAGCGGAT GATTCTGCGC 301 TTGCAGCAAG AGTATCCGGA ACTAGCAGGT CAAAATCACT GAACGAAGCA ACTGTTAGAG 361 GGTTGAGCAC AACAAGGCAT CTTGTTGTTA AATGGCGTCT TTACCACCAA TGCCTTTCAA 421 ACCAATGCGC ACCGCAATTG GGGGTGAGAG CAGTTTAACT GTCATTTACT CGATTTTCTC 481 TTGTCACAAA AACTCTATGT ACTACTCGTT TTTTTGGGCA AACAAACCGA GAATTTTGTT 541 CTTAAAAAGA TTGGTGGAGC AAGCCAATTT GCTAGTTTAT CCTACCCCCA TCCCTCACCT 601 TTAACCGGGC GGAAGTTTTT CGATCATCCC GATGCTTTGT TTAAGCAAAT TAACCAGTGA 661 TTAAAGCACC ATAACCATAC TCCAATTGAT TGGACTAACG GCCAGGTACA GTACCAGTTC 721 TAA

MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACG

LSFASNAPKLPQSLKRMILRLQQEYPELAGQNHWTKQLLEGWAQQGILLLNGVFTTNAFQTNAHRNWGWEQFNCHLLDFL

LSQKLYVLLVFLGKQTENFVLKKIGGASQFASLSYPHPSPLTGRKFFDHPDALFKQINQWLKHHNHTPIDWTNGQVQYQF

Location (aa)	Name (InterPro ID)	Description	GO terms	Sequence	Evalue
13-226	TIGR00628 (IPR002043)	Uracil-DNA glycosylase	Biological Process: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006284'>'base' == '' ? '': 'base';-'excision' == '' ? '': 'excision'; 'repair' == '' ? '': 'repair'; ('GO' == '' ? '': 'GO';:0006284) Molecular Function: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0004844'>'uracil' == '' ? '': 'uracil'; 'DNA' == '' ? '': 'DNA'; 'N' == '' ? '': 'N';-'glycosylase' == '' ? '': 'glycosylase'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0004844)		0
14-232	G3DSA:3.40.470.10 (IPR005122)	Uracil-DNA glycosylase-like			0
14-240	SSF52141 (IPR005122)	Uracil-DNA glycosylase-like			0
29-237	PTHR11264 (IPR002043)	Uracil-DNA glycosylase	Biological Process: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006284'>'base' == '' ? '': 'base';-'excision' == '' ? '': 'excision'; 'repair' == '' ? '': 'repair'; ('GO' == '' ? '': 'GO';:0006284) Molecular Function: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0004844'>'uracil' == '' ? '': 'uracil'; 'DNA' == '' ? '': 'DNA'; 'N' == '' ? '': 'N';-'glycosylase' == '' ? '': 'glycosylase'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0004844)		1.9e-25
57-222	PF03167 (IPR005122)	Uracil-DNA glycosylase-like			0.00000000000067
63-72	PS00130 (IPR018085)	Uracil-DNA glycosylase, active site	Biological Process: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0006281'>'DNA' == '' ? '': 'DNA'; 'repair' == '' ? '': 'repair'; ('GO' == '' ? '': 'GO';:0006281) Molecular Function: <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:'hydrolase' == '' ? '': 'hydrolase';'>'hydrolase' == '' ? '': 'hydrolase'; 'activity' == '' ? '': 'activity';		0

Name	ung
Stable ID	MPN235
Location	286468 - 287190 +
Sequence	1 ATGGAACAAC TTTTAGACCA GTTTTTAAGT GGTGTTTTAA GCGAATGGAA GGCTTTTATT 61 TTGCAAGAAG CCAAACAGAC CTATTTTCAA GAATTGTTAG CTAAACTAAA AAATGTCGAG 121 CAAGAGCTAA CTCCCAAAGC CTCTCAAGTC TTTCGTCCCT TTAGCTTTTT TGCACCAAAT 181 AACACTAAAC TAATTATTTA CGGTCAGGAT CCATATCCTA ATCCACAACA CGCTTGTGGT 241 CTTTCGTTTG CCTCTAACGC CCCTAAACTA CCACAAAGCT TAAAGCGGAT GATTCTGCGC 301 TTGCAGCAAG AGTATCCGGA ACTAGCAGGT CAAAATCACT GAACGAAGCA ACTGTTAGAG 361 GGTTGAGCAC AACAAGGCAT CTTGTTGTTA AATGGCGTCT TTACCACCAA TGCCTTTCAA 421 ACCAATGCGC ACCGCAATTG GGGGTGAGAG CAGTTTAACT GTCATTTACT CGATTTTCTC 481 TTGTCACAAA AACTCTATGT ACTACTCGTT TTTTTGGGCA AACAAACCGA GAATTTTGTT 541 CTTAAAAAGA TTGGTGGAGC AAGCCAATTT GCTAGTTTAT CCTACCCCCA TCCCTCACCT 601 TTAACCGGGC GGAAGTTTTT CGATCATCCC GATGCTTTGT TTAAGCAAAT TAACCAGTGA 661 TTAAAGCACC ATAACCATAC TCCAATTGAT TGGACTAACG GCCAGGTACA GTACCAGTTC 721 TAA
Download Sequence
Operon	OP96
Operon location	284500 - 287190

Name	Uracil-DNA glycosylase (UDG) (EC 3.2.2.-)
Stable ID	Mpn235
Molecular Weight	26400
Isoelectric Point	9
Localization	Cytoplasm
Comment	Base excision repair; All DNA containing organisms contain a specific repair pathway which removes uracil from DNA. Uracil-DNA glycosylase (UDGase) catalyses the first step in this pathway, hydrolysing the N-glycosidic bond connecting the base to the deoxyribose sugar
Sequence	MEQLLDQFLSGVLSEWKAFILQEAKQTYFQELLAKLKNVEQELTPKASQVFRPFSFFAPNNTKLIIYGQDPYPNPQHACG LSFASNAPKLPQSLKRMILRLQQEYPELAGQNHWTKQLLEGWAQQGILLLNGVFTTNAFQTNAHRNWGWEQFNCHLLDFL LSQKLYVLLVFLGKQTENFVLKKIGGASQFASLSYPHPSPLTGRKFFDHPDALFKQINQWLKHHNHTPIDWTNGQVQYQF

Modification	Modified sequence	Relative start	Relative end	Amino acid
Oxidation	mEQLLDQFLSGVLSEWK	1	18	M

Protein Start	Protein End	Hit Size	Orthologous	Organism	Orthologous Start	Orthologous End	Evalue	Identity	Score	Alignment
16	231	215	- b2580	Escherichia coli	7	220	5e-26	33.7778	277
16	232	216	- MARTH_orf815	Mycoplasma arthritidis	5	216	4e-30	33.79	306
19	232	213	- MAG0680	Mycoplasma agalactiae	8	214	1e-27	33.1818	285
13	232	219	- MCJ_001880	Mycoplasma conjunctivae	9	223	3e-30	34.2342	307
29	232	203	- MCAP_0474	Mycoplasma capricolum subsp. capricolum	21	217	2e-24	32.8502	259
4	232	228	- MYCGA6090	Mycoplasma gallisepticum	1	227	9e-21	30.3797	226
1	232	231	- MG097	Mycoplasma genitalium	3	234	<1e-50	56.0345	713
16	232	216	- MHP7448_0130	Mycoplasma hyopneumoniae 7448	7	218	3e-32	32.8767	325
19	232	213	- MHO_4330	Mycoplasma hominis	9	213	2e-33	39.7196	334
18	231	213	- MMOB0720	Mycoplasma mobile	13	220	6e-26	32.093	270
29	206	177	- MSC_0496	Mycoplasma mycoides subsp. mycoides SC	21	192	9e-19	31.4917	209
39	231	192	- MYPE2070	Mycoplasma penetrans	58	246	5e-21	31.8182	230
14	232	218	-	Lactococcus lactis subsp. lactis KF147	4	218	2e-21	30.9735	235
22	231	209	- MS53_0307	Mycoplasma synoviae	11	214	3e-32	36.3208	325
16	231	215	- Rv2976c	Mycobacterium tuberculosis	14	224	7e-21	32.4201	233
22	232	210	-	Bacillus subtilis subsp. subtilis	15	221	3e-26	33.3333	281
22	232	210	-	Bacillus subtilis subsp. subtilis	15	221	4e-26	33.3333	280
16	232	216	-	Mycoplasma pulmonis	7	219	3e-27	33.6364	283

COG	COG0692L
EC number	3.2.2.-
Gene ID	877251
GI	13507974
GO	DNA replication, recombination and repair
Home COG	L
InterPro	IPR003249\|Uracil-DNA glycosylase, not poxvirus
InterPro	IPR002043\|Uracil-DNA glycosylase
InterPro	IPR005122\|Uracil-DNA glycosylase superfamily
Old MP number	MP596
Pathway	DNA repair and maintenance
PDB homologs	1eug_A
PDB homologs	1lqj_A
PDB homologs	1lqg_A
PDB homologs	1LAU-E
Pfam	PF03167
PID	g1674298
RefSeq	NP_109923.1
Swiss-Prot protein ID	UNG_MYCPN
phylomeDB tree	UNG_MYCPN
UniProt	P75536