MyMpn
The Mycoplasma pneumoniae database
 
e.g Mpn001 or dnaN or polymerase
Gene polA (MPN379)
Name 
polA
Stable ID 
MPN379
Location 
456272 - 457147 +
Sequence 
    1  ATGAAAAATG CAATTTTAAT TGATGGTAAC TCACTAGCCT ACCGTGCTTA CTTCGCTACT
   61  TGACAACAGG TGGAGTTTGC CAAACTACAC AACTTACCCT TTAACAATGC GATTCGCACA
  121  ATGTTAATGA TGTGCTGAAA CTTACTGCAG TCAAAACAGT ATGACTATGG TGTTATTAGC
  181  TTTGATACTA AAGCGCCCAC TTTTCGTGAT CAACTTTACA GTGAATACAA AAGTAAGCGC
  241  AGTAAAACAC CGAGTGAACT GTTAGTCCAA ATTCCTGTTG TTAAAGAATC ACTGCGTCAT
  301  TTAGGTTTTT TAGTGTGTGA ACAAGACGGG TTTGAGGCAG ATGATCTAAT TGGTAGTTAT
  361  GCGAGGTTAA TGACACAAAA CAATGTGGCA GTAGACATCT ACAGCTCTGA CCGTGATTTA
  421  CTACAGTTAG TCGATAGTAT GACTAGTGTT TGGCTTTGTG TAAAGGGCAC AAAAGAGATG
  481  AAGGAATATA ACACTGACAA CTTTGCTGAG CAGTTTTTTG GCTTAACACC AAAACAAGTG
  541  ATTGAATATA AAGGTTTGGT AGGGGATAAC AGTGATAACC TCACTGGCAT TAAGGGAATT
  601  GGTCCGAAAA AAGGGATTGA TCTACTCAAA CAATACGGTA CCATTGACAA TATTTTTGCT
  661  AACTTTGACA AGCTATCCAA AGCATTGCAA ACGATTCTCC AAGGCCAAAT TGACACTGCT
  721  AAGAAATTCA GCTTTTTAGC GAGCATTAAA ACAGATATTA AACTTAATGA CGATATTGTC
  781  CACGCTGCTT TAAAACCAAT CGATAAACAA GCTTTATTAG AGCTTTTAGA TAAATATGGC
  841  ATTAAAGCAC TAGCGCAAAA ATTTAGTCAA CTGTAA
Download Sequence
Operon 
OP152
Operon location 
453392 - 457035
Protein (mpn379)
Name 
5'-3' exonuclease (EC 3.1.11.-)
Stable ID 
Mpn379
Molecular Weight 
32010
Isoelectric Point 
8
Localization 
Cytoplasm
Comment This genes is only part of B. subtilis polA, it lacks the polymerase and proff-reading domains. However, this gene is dispensible in E.coli and B. subtilis as long as there is an 5'-3' exonuclease activity, indicating that just this activity (in cooperation with RnaseH) is involved in the lagging strand gap filling (J Bacteriol, 2007, 189:8575)
Sequence 
MKNAILIDGNSLAYRAYFATWQQVEFAKLHNLPFNNAIRTMLMMCWNLLQSKQYDYGVISFDTKAPTFRDQLYSEYKSKR
SKTPSELLVQIPVVKESLRHLGFLVCEQDGFEADDLIGSYARLMTQNNVAVDIYSSDRDLLQLVDSMTSVWLCVKGTKEM
KEYNTDNFAEQFFGLTPKQVIEYKGLVGDNSDNLTGIKGIGPKKGIDLLKQYGTIDNIFANFDKLSKALQTILQGQIDTA
KKFSFLASIKTDIKLNDDIVHAALKPIDKQALLELLDKYGIKALAQKFSQL
Post translational modifications
Modification Modified sequence Relative start Relative end Amino acid
Oxidation mKNAILIDGNSLAYR 1 16 M
Oxidation GTKEmKEYNTDNFAEQFFGLTPK 156 179 M
Methylation kGIDLLK 204 211 K
GENE/PROTEIN polA (Domains Overview)
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 ExportIMG
Domains (InterProScan)
Location (aa) Name (InterPro ID) Description GO terms Sequence Evalue
1-172 SSF88723 (n.a.) NULL 0
2-265 SM00475 (IPR002421) 5'-3' exonuclease, N-terminal Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003677'>'DNA' == '' ? '': 'DNA'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003677) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008409'>5'-3' 'exonuclease' == '' ? '': 'exonuclease'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0008409)

0
3-172 PF02739 (IPR020046) 5'-3' exonuclease, alpha-helical arch, N Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003677'>'DNA' == '' ? '': 'DNA'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003677) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008409'>5'-3' 'exonuclease' == '' ? '': 'exonuclease'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0008409)

0
3-186 G3DSA:3.40.50.1010 (n.a.) NULL 0
6-288 PTHR10133 (n.a.) NULL 0
175-273 PF01367 (IPR020047) 5'-3' exonuclease, SAM-fold domain Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003677'>'DNA' == '' ? '': 'DNA'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003677) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0008409'>5'-3' 'exonuclease' == '' ? '': 'exonuclease'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0008409)

1.2e-27
175-291 SSF47807 (IPR020045) 5'-3' exonuclease, C-terminal subdomain Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003677'>'DNA' == '' ? '': 'DNA'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003677) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003824'>'catalytic' == '' ? '': 'catalytic'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0003824)

8.49999e-29
177-212 SM00279 (IPR008918) Helix-hairpin-helix motif, class 2 Molecular Function:
<'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003677'>'DNA' == '' ? '': 'DNA'; 'binding' == '' ? '': 'binding'; ('GO' == '' ? '': 'GO';:0003677) <'A' == '' ? '': 'A'; 'class' == '' ? '': 'class';=''result' == '' ? '': 'result';' 'target' == '' ? '': 'target';='_'blank' == '' ? '': 'blank';' 'href' == '' ? '': 'href';=''http' == '' ? '': 'http';://'amigo' == '' ? '': 'amigo';.'geneontology' == '' ? '': 'geneontology';.'org' == '' ? '': 'org';/'cgi' == '' ? '': 'cgi';-'bin' == '' ? '': 'bin';/'amigo' == '' ? '': 'amigo';/'term' == '' ? '': 'term';-'details' == '' ? '': 'details';.'cgi' == '' ? '': 'cgi';?'term' == '' ? '': 'term';='GO' == '' ? '': 'GO';:0003824'>'catalytic' == '' ? '': 'catalytic'; 'activity' == '' ? '': 'activity'; ('GO' == '' ? '': 'GO';:0003824)

0.00000000000072
187-241 G3DSA:1.10.150.20 (n.a.) NULL 2.6e-21
Protein Homology (BLASTP results)
Protein Start Protein End Hit Size Orthologous Organism Orthologous Start Orthologous End Evalue Identity Score Alignment
5 259 254 - MAG1650 Mycoplasma agalactiae 9 266 1e-28 29.6578 295
1 273 272 - MARTH_orf613 Mycoplasma arthritidis 1 261 1e-38 34.1818 381
6 287 281 - Caulobacter crescentus NA1000 45 322 2e-27 28.9199 290
5 274 269 - MCJ_005390 Mycoplasma conjunctivae 6 286 3e-25 29.9296 266
5 255 250 - MHO_4130 Mycoplasma hominis 9 255 1e-28 32.9365 294
5 225 220 - Lactococcus lactis subsp. lactis KF147 8 229 1e-39 37.2197 393
2 290 288 - Bacillus subtilis subsp. subtilis 5 289 2e-37 30.6897 378
5 291 286 - Bacillus subtilis subsp. subtilis 7 290 8e-31 30.3136 321
5 291 286 - Bacillus subtilis subsp. subtilis 7 290 8e-31 30.3136 321
2 290 288 - Bacillus subtilis subsp. subtilis 5 289 2e-37 30.6897 379
5 282 277 - b3863 Escherichia coli 10 285 2e-26 28.8136 281
5 288 283 - Rv1629 Mycobacterium tuberculosis 18 298 1e-33 31.338 343
28 259 231 - b2798 Escherichia coli 16 244 0.000000000000002 22.6891 187
5 283 278 - Rv2090 Mycobacterium tuberculosis 6 304 0.000000000000003 24.918 185
1 291 290 - MCAP_0636 Mycoplasma capricolum subsp. capricolum 1 294 1e-37 33.0033 373
2 250 248 - MCAP_0047 Mycoplasma capricolum subsp. capricolum 7 258 7e-29 28.8538 297
1 291 290 - MG262 Mycoplasma genitalium 1 291 <1e-50 64.6048 1019
1 223 222 - MS53_0528 Mycoplasma synoviae 5 220 1e-27 34.0708 286
2 257 255 - MHP7448_0581 Mycoplasma hyopneumoniae 7448 3 251 2e-25 33.5907 268
1 288 287 - MMOB4880 Mycoplasma mobile 3 289 7e-32 30.7432 322
1 291 290 - MSC_0683 Mycoplasma mycoides subsp. mycoides SC 1 294 5e-37 32.5581 368
2 250 248 - MSC_0091 Mycoplasma mycoides subsp. mycoides SC 7 258 3e-27 29.249 283
1 256 255 - LIC_10586 Leptospira interrogans serovar Copenhage 1 256 6e-27 29.0698 285
2 284 282 - MYCGA2330 Mycoplasma gallisepticum 5 287 <1e-50 36.7491 481
2 279 277 - MYPE1990 Mycoplasma penetrans 3 281 <1e-50 39.7849 512
5 290 285 - Mycoplasma pulmonis 12 288 1e-20 25.8503 227
External IDs
COG 
COG0258L
EC number 
3.1.11.-
Gene ID 
877399
GI 
13508118
GO 
DNA replication, recombination and repair
Home COG 
L
InterPro 
IPR002421|5'-3' exonuclease
InterPro 
IPR008918|Helix-hairpin-helix motif, class 2
Old MP number 
MP458
Pathway 
DNA degradation 
PDB homologs 
1tau_A
PDB homologs 
1bgx_T
PDB homologs 
1cmw_A
PDB homologs 
1BGX-T
Pfam 
PF02739
Pfam 
PF00168
Pfam 
PF08830
Pfam 
PF01367
PID 
g1674147
RefSeq 
NP_110067.1
Swiss-Prot protein ID 
EX53_MYCPN
phylomeDB tree 
EX53_MYCPN
UniProt 
P75403
Transcription
IMAGE BROWSERS

OPERON OP152 (Genomic Overview)
Region:453392-457035

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STRING image

STRING of Mpn379STRING legend

PDB image(s)

1tau

PDB 1tau

1bgx

PDB 1bgx

1cmw

PDB 1cmw

1BGX

PDB 1BGX
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All data provided for download in the MyMpn database is free to use. Upon doing so, please cite the original data source and the MyMpn database
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